Volume 3, Issue 6, December 2017, Page: 45-55
Development and Validation for HPLC Method of Assay of Lvermectin and Clorsulon in Combined Pharmaceutical Dosage Form
Mahmoud Mohamed Ali, Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa., Khartoum, Sudan
Elfatih Elbashir, Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa., Khartoum, Sudan
Mohamed N. Abdalaziz, Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa., Khartoum, Sudan
Received: Sep. 15, 2017;       Accepted: Nov. 6, 2017;       Published: Jan. 16, 2018
DOI: 10.11648/j.ijhnm.20170306.11      View  1791      Downloads  203
Abstract
Stability indicating-HPLC method has been developed for simultaneous estimation of Ivermectin and Clorsulon in their combined dosage form. For RP-HPLC method, all the standard and sample solutions were prepared in methanol. A RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Ivermectin and Clorsulon in their combination product. The proposed RP-HPLC method utilizes a Thermo BDS C-18 (15cm x 4.6mm, 5 μm) column, mobile phase consisting of acetonitrile, methanol and purified water in the proportion of 60: 30:10 (v/v/v), and UV detection at 245 nm. The described method was linear over a range of 10-40μg/ml with a correlation coefficient (r2) of 0.9998 for Ivermectin and a range of 100-400μg/ml with a correlation coefficient (r2) of 0.9998 for Clorsulon. Validations of the proposed method were carried out for its accuracy, precision, linearity and range, specificity, LOD and LOQ according to ICH guidelines. A stability-indicating study was also carried out and indicated that this method can also be used for purity and degradation evaluation of these formulations that occurred due to temperature, humidity and time. the method has been successfully applied for the analysis of drugs in formulation.
Keywords
Ivermectin, Clorsulon, RP-HPLC, Limit of Detection, Limit of Quantitation
To cite this article
Mahmoud Mohamed Ali, Elfatih Elbashir, Mohamed N. Abdalaziz, Development and Validation for HPLC Method of Assay of Lvermectin and Clorsulon in Combined Pharmaceutical Dosage Form, International Journal of Homeopathy & Natural Medicines. Vol. 3, No. 6, 2017, pp. 45-55. doi: 10.11648/j.ijhnm.20170306.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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